Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21/pBBNH cells | Bacillus badius |
General Stability | Organism |
---|---|
glycosylation increases temperature stability | Bacillus badius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.186 | - |
L-Phe | PheDH after glycosidation | Bacillus badius | |
0.215 | - |
L-Phe | unmodified PheDH | Bacillus badius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
326000 | - |
SDS-PAGE, unmodified PheDH | Bacillus badius |
720000 | - |
SDS-PAGE, neoglyoenzyme | Bacillus badius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus badius | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the neoglycoenzyme retains 63% of its initial activity and contains about 2.5 mol of O-carboxymethyl poly-beta-cyclodextrin per mole of enzyme | Bacillus badius |
Purification (Comment) | Organism |
---|---|
Ni-affinity and anion exchange column chromatography | Bacillus badius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
450 | - |
PheDH after glycosidation | Bacillus badius |
816 | - |
unmodified PheDH | Bacillus badius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Phe + H2O + NAD+ | - |
Bacillus badius | phenylpyruvate + NH3 + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PheDH | - |
Bacillus badius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
unmodified PheDH | Bacillus badius |
55 | - |
neoglycoenzyme | Bacillus badius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | - |
Bacillus badius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 61 | the optimum temperature for the glycosylated enzyme is increased by 15°C and its thermostability is improved by about 6°C over 10 min incubation, the conjugate is more resistant to thermal inactivation at different temperatures, ranging from 45 to 60°C, the T50 value is increased from 54 to 61°C for PheDH after glycosidation | Bacillus badius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus badius |